Holy Trinity Cathedral, is the seat of the Church of Pakistan, Diocese of Karachi,  situated on Fatima Jinnah Road, near Zainab Market, in Karachi, Pakistan. The current Bishop is The Rt. Rev. Sadiq Daniel. There is also a Theological College in the grounds of the Cathedral.

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Built in 1855, Holy Trinity Cathedral was designed by Captain John Hill of the Bombay Engineers. This Cathedral was one of the first major churches of Karachi. Its tall tower was also a light house as it was built with a purpose to facilitate the ships arriving at Karachi Harbour.